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. 2022 Mar 10;29(9):1689–1704. doi: 10.1038/s41418-022-00956-7

Fig. 1. Genome-scale screening of the ubiquitin-specific protease subfamily for PAX9 and MSX1 proteins using CRISPR-Cas9-based DUB-knockout library kit.

Fig. 1

A Screening for USPs regulating PAX9 and MSX1 proteins using the CRISPR-Cas9-based DUB KO library kit. Equal protein concentrations from DUB KO HEK293 cell lysates were subjected to Western blotting to determine endogenous PAX9 and MSX1 protein levels. GAPDH was used as a loading control for each blot. Asterisk represents the selected DUB, which regulates both PAX9 and MSX1 proteins. The presented immunoblots are representative of three independent experiments (n = 3). B Effects of putative DUB candidates (USP11, USP13, and USP49) on PAX9 and MSX1 protein levels in HEK293 cells were estimated by Western blotting. The presented immunoblots are representative of three independent experiments (n = 3). C Effects of putative DUB candidates (USP11, USP13, and USP49) on PAX9 and MSX1 protein levels in hDPSCs were estimated by Western blotting. The presented immunoblots are representative of three independent experiments (n = 3).