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. 2022 Mar 8;29(9):1705–1718. doi: 10.1038/s41418-022-00957-6

Fig. 1. High expression of HUWE1 correlates with less hepatic injury in liver transplantation patients.

Fig. 1

A, B The peri-operative graft expression of HUWE1 protein levels were detected from a human liver transplantation (LT) cohort (A, n = 14). (B) Protein levels of HUWE1 were normalized to those of GAPDH (pre-LT: 1 ± 0.68 vs post-LT: 0.58 ± 0.42). C HUWE1 mRNA levels in hepatic biopsies from another human liver transplantation cohort (n = 11, pre-LT: 1.41 ± 2.06 vs post-LT: 0.50 ± 0.44). D Immunohistochemistry of HUWE1 in hepatic biopsies from a human liver transplantation cohort (n = 6). Scale bar, 50 μm. E, F Huwe1 protein levels in the livers of mice subjected to 90min of ischemia and subsequent reperfusion for 6 or 24 h. Gapdh served as a loading control. n = 3-5 per time point. Huwe1 protein levels were normalized to those of Gapdh (F). G–J From a human liver transplantation cohort (n = 27), HUWE1 expression was detected by Western Blots in donor liver samples (G). Pre-LT HUWE1 was analyzed with normalization to GAPDH (H). MDA were detected in LT recipients’ liver biospies (I, high: 1.21 ± 1.02 vs low: 3.19 ± 2.94). J The peak serum ALT (left) and AST (right) were measured within 48 h in LT recipients after liver transplantation (ALT, high: 326.3 ± 176 vs low: 526.9 ± 253.8; AST, high: 463.4 ± 206.3 vs low: 696.5 ± 354.9). The error bars represent the standard deviation of the indicated dataset. p value in (B) and (C) was analyzed by paired Student’s t test (Prism; GraphPad) and the rest was analyzed by unpaired two-tailed Student’s t test. **p < 0.01, ****p < 0.0001 compared with sham group.