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. 2022 Feb 14;43(9):2397–2409. doi: 10.1038/s41401-022-00862-1

Fig. 1. PTEN-deficient ccRCC cell lines are more sensitive to everolimus and temsirolimus than PTEN-proficient ccRCC cell lines.

Fig. 1

a Immunoblots showing relevant protein expression in 13 ccRCC cell lines. * indicates nonspecific bands. b Viability assay in ccRCC cell lines after treatment with everolimus or temsirolimus at the indicated concentrations for 6 days. The data are presented as the mean ± SD (n = 3) of one representative experiment out of three (left). The EC50 values for each cell line are presented as the mean ± SD (n = 3) of three separate experiments (right). c Representative immunohistochemical (IHC) images of PTEN expression in paraffin-embedded tissues from patients with ccRCC and adjacent normal tissues are shown. Scale bar: 50 μm (left). The percentage of PTEN-positive and PTEN-negative specimens among 145 ccRCC samples (right).