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. 2022 Feb 14;43(9):2397–2409. doi: 10.1038/s41401-022-00862-1

Fig. 2. PTEN deletion renders HEK293A cells more sensitive to rapalogs.

Fig. 2

VHL (a) PBRM1 (b), BAP1 (c), SETD2 (d), KDM5C (e), and PTEN (f) knockout clones were validated by Western blotting. Cells were treated with temsirolimus or everolimus at the indicated concentrations for 6 days, and an MTT assay was used to determine cell viability. The data are presented as the mean ± SD (n = 3) of one representative experiment out of three.