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. 2022 Aug 18;12:953168. doi: 10.3389/fcimb.2022.953168

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Copyright © 2022 Atiencia-Carrera, Cabezas-Mera, Vizuete, Debut, Tejera and Machado

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Illustration of the biofilms of C. albicans and C. tropicalis at 48 and 72 h of growth by epifluorescence microscopy using 4′,6-diamidino-2-phenylindole fluorescent stain and LIVE/DEAD Biofilm Viability Kit. Time samples of 48 and 72 h were used to compare the total cell and live/dead cells in the biofilms using an Olympus BX50 microscope, and pictures were obtained by AmScope software at ×100 magnification. Then, the pictures from each filter were merged in Fiji-ImageJ version 1.57 (Schindelin et al., 2012).