Table 2.
Evaluation of the statistical differences in biofilm growth between Candida albicans and Candida tropicalis through biomass and viability assays.
| Biofilm growth | C. albicans | C. tropicalis | Non-parametrical statistical analysis (interspecies) | |||||
|---|---|---|---|---|---|---|---|---|
| Assays | n a | Mean | SD | n a | Mean | SD | Wilcoxon test p-value | |
| Biomass PBS A630 | ||||||||
| 24 h | 4 | 0.280 | 0.127 | 4 | 0.667 | 0.160 | 4.81E - 07 | |
| 48 h | 3 | 0.562 | 0.184 | 3 | 0.936 | 0.126 | 1.16E - 05 | |
| 72 h | 3 | 0.822 | 0.169 | 3 | 1.170 | 0.090 | 1.44E - 05 | |
| 96 h | 3 | 1.045 | 0.109 | 3 | 1.290 | 0.130 | 3.99E - 05 | |
| Biomass CV A630 | ||||||||
| 24 h | 4 | 0.056 | 0.014 | 4 | 0.151 | 0.018 | 5.44E - 08 | |
| 48 h | 3 | 0.168 | 0.015 | 3 | 0.270 | 0.026 | 5.54E - 07 | |
| 72 h | 3 | 0.269 | 0.011 | 3 | 0.328 | 0.038 | 7.00E - 04 | |
| 96 h | 3 | 0.314 | 0.031 | 3 | 0.338 | 0.045 | 4.33E - 02 | |
| Viability CFU/ml | ||||||||
| 24 h | 4 | 1.22E + 08 | 2.23E + 07 | 4 | 9.89E + 08 | 1.60E + 08 | 6.67E - 08 | |
| 48 h | 3 | 2.49E + 08 | 4.59E + 07 | 3 | 2.33E + 09 | 2.79E + 08 | 6.17E - 07 | |
| 72 h | 3 | 4.13E + 08 | 1.09E + 08 | 3 | 2.99E + 09 | 3.09E + 08 | 3.37E - 06 | |
| 96 h | 3 | 4.77E + 08 | 3.05E + 07 | 3 | 5.58E + 09 | 8.22E + 08 | 3.27E - 06 | |
The standard deviation (SD) and the mean were calculated by the average values of the total number of assays (five samples per assay). The data set showed a non-normal distribution among the results, and consequently, a non-parametrical statistical analysis was selected to evaluate statistical differences in biofilm growth between C. albicans and C. tropicalis. More specifically, the results between Candida species were evaluated using the Wilcoxon test. aNumber of assays realized on different days for each time sample.