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. 2022 Aug 18;13:922173. doi: 10.3389/fimmu.2022.922173

Figure 1.

Figure 1

Macrophage accumulation and polarization in human IVD tissues. (A) Representative MR images showing normal (Pfirrmann grade I, n = 5) and IDD samples (Pfirrmann grades III-V, n = 8), respectively. (B) Representative images showing the immunolocalization of CCR7-positive cells in human normal IVD tissues; (C) Comparison of the proportion of positive cells corresponding to normal and degenerated IVD samples. (D) Representative images showing the immunolocalization of CD206-positive cells in human IDD tissues, and (E) Comparison of positive cells corresponding to normal and IDD samples. The data were analyzed using Mann–Whiney U test. **p < 0.01; ***p < 0.001. Data are shown as mean ± SD. IVD, intervertebral disc; IDD, intervertebral disc degeneration. (F) Flow diagram of harvesting conditioned medium (CM) and grouping process of CM in vitro and in vivo.