Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 18;13:922173. doi: 10.3389/fimmu.2022.922173

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Li, Luo, Fan, Zhou, Tan, Tan, Xian, Li, Huang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Effect of macrophage polarization on ECM synthesis and pro-inflammatory mediator secretion in TNF-α-treated NPCs. (A) Representative images showed collagen II immunofluorescence staining results corresponding to NPCs in different groups. (B) Comparison of the OD values corresponding to different test compounds after 7 days of treatment. (C, D) WB analysis of anabolic aggrecan and catabolish MMP-13in NPCs treated with different CMs for 7 days. (E–L) Gene expression of ECM components (aggrecan, collagen IIα1, and collagen Iα1), ECM-modifying enzymes (MMP-13), and pro-inflammatory mediators (IL-1β, IL-6, IL-8, and IL-12) in TNF-α-treated NPCs after 3 days based on qPCR. All data are expressed as the mean ± SD, n = 3, *P < 0.05, **P < 0.01. NPCs, nucleus pulposus cells; TNF-α, tumor necrosis factor-α; CM, condition medium; ECM, extracellular matrix; OD, optical density.