TABLE 1.
Plasmids and proteins used in this study
| Plasmid | Protein | Size (kDa) | Feature | Reference or source |
|---|---|---|---|---|
| pMLG43 | GbpA | 59.0 | Native protein, secreted by S. mutans | 21 |
| GBD | 47.8 | GBD from GBP-A (unless noted otherwise) | ||
| PinPoint Xa1 | TCS | 13.1 | TCS from P. shermanii, biotinylated in vivo | Promega |
| pDPL4 | B-GBD | 60.9 | Fusion protein between TCS and GBD from GbpA, 16 YG repeats, biotinylated | This study |
| pDPL-B | B-GBD-B | 75.5 | Same as B-GBD, but TCS at both N and C termini | This study |
| pDPL4-78 | B–GBD-14n | 53.8 | Same as B-GBD, but two YG repeats deleted from the N terminus | This study |
| pDPL-108 | B–GBD-10n | 42.6 | Same as B-GBD, but six YG repeats deleted from the N terminus | This study |
| pDPL-14c | B–GBD-14c | 55.6 | Same as B-GBD, but two YG repeats deleted from the C terminus | This study |
| pDPL-10c | B–GBD-10c | 42.9 | Same as B-GBD, but six YG repeats deleted from the C terminus | This study |
| pKM2 | SKC | 47 | SKC from Streptococcus equisimilis | 12 |
| pGS1 | GBD-SKC | 95 | Fusion between GBD at the N terminus and SKC at the C terminus | This study |
| pGB | B-GB | 60.8 | Fusion between TCS and the GBD from GTF-I from S. mutans (similar to B-GBD) | This study |
| pGD | B-GD | 62.4 | Fusion between TCS and the GBD from GTF-S from S. mutans (similar to B-GBD) | This study |