FIG. 2.

Sequence of the region upstream of the cry1Acl gene from the NsiI site to the ATG initiation codon. The −10 and −35 regions of the dual overlapping promoters are singly (BtI) or doubly (BtII) underlined, with the base substitutions in the −10 regions for the 271 (BtI) and 272 (BtII) oligonucleotides indicated below each. The start sites of transcription are marked (I and II), and the ribosome binding site is overlined. The sequence of an additional 900 bp upstream of the cry1A genes is available (GenBank accession number AF039908).