FIG. 5.

Reverse transcriptase mapping of the start sites of transcription from the wild-type (Wt) and mutant (271 and 272) promoters in plasmid pHT/pSGMU-P in B. thuringiensis 80-21. The start sites for BtI and BtII are shown on the right. The Wt RNA was prepared from cells at the equivalent of 14 to 15 h in Fig. 4, the RNA for the 271 lane was prepared from cells at the equivalent of 16 to 17 h in Fig. 4, and the RNA for the 272 lanes (separate gel) was prepared from cells at the equivalent of 16 and 17 h in Fig. 4. All of the sequencing lanes are in the order AGCT as indicated in the panel on the right side. Note that these sequencing lanes start six bases lower than the Wt and 271 sequencing lanes and that this sequence is compressed at the top.