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Northern hybridizations of 20 μg of RNA prepared from CryB/pHT3101-1Acwt (lanes 1 and 4) and CryB/pHT3101-1Ac272 (lanes 2 and 3) at T2.5 (lanes 1 and 2) and T6 (lanes 3 and 4) of sporulation. Hybridization and quantitation of both bands were done as described in Materials and Methods. The upper bands in each lane are the expected size for cry1Ac1 mRNA; the lower, less prevalent bands are apparently stable degradation products.
