FIG. 7.
(A) Immunoblot of cell extracts of strains CryB/pHT3101-1Acwt (lanes 1 to 3) and CryB/pHT3101-1Ac272 (lanes 4 to 6) sampled at T1.5 (lanes 1 and 4), T3.0 (lanes 2 and 5), and T4.5 (lanes 3 and 6). Electrophoresis and treatment with a Cry1Ac antibody were done as described in Materials and Methods. (B) Stained SDS–10% PAGE of crude spore-inclusion mixtures (lanes 1 and 2) and of purified inclusions (lanes 3 and 4) from CryB/pHT3101-1Acwt (lanes 1 and 3) and CryB/pHT3101-1Ac272 (lanes 2 and 4). Standards (STD), from top to bottom, are 116, 97, 66, 45, and 29 kDa.