FIG. 9.

Autoradiogram of SDS-PAGE of immunoprecipitated Cry1Ac antigen from CryB/pHT3101-1Acwt (lanes 1 to 4) and CryB/pHT3101-1Ac272 (lanes 7 to 10) from a pulse-chase experiment (see Materials and Methods). Five-milliliter aliquots of the cultures were removed and incubated with [¹⁴C]isoleucine for 7 min at clumping (about T_0.5) (lanes 1 and 7) and then chased with an excess of [¹²C]isoleucine for 1 h (lanes 2 and 8). At this time (T_1.5), another 5-ml sample of cells was incubated with [¹⁴C]isoleucine for 7 min (lanes 3 and 9) and chased (lanes 4 and 10). Lanes 5 and 6 contained extracts, like lanes 1 and 2, but they were treated only with preimmune serum.