Figure 10.

Lower olfactory dishabituation to social odors and intact olfactory-guided behavior in Scn2a^E/+ mice. (A) Average sniffing times during an odor habituation/dishabituation assay in Scn2a^E/+ mice compared with WT at 11 weeks of age. Overall, olfactory discrimination in Scn2a^E/+ males was not significantly different from WT males. However, Scn2a^E/+ males spent significantly less time sniffing same sex urine during the first two presentations compared with WT males. During the first presentation, average sniffing time was 42.3 ± 3.3 s for WT males and 18.8 ± 3.4 s for Scn2a^E/+ males (^***P = 0.0005, multiple t-tests). During the second presentation, average sniffing time was 26.3 ± 8.9 s for WT males and 8.9 ± 2.1 s for Scn2a^E/+ males (^*P = 0.0307, multiple t-tests). Olfactory discrimination in Scn2a^E/+ females was also not significantly different from WT females. However, Scn2a^E/+ females spent significantly less time sniffing same sex urine during the first presentation compared with WT females (WT: 46.3 ± 4.6 s, Scn2a^E/+: 19.9 ± 4.2 s, ^**P = 0.0045, multiple t-tests). Symbols and error bars represent mean ± SEM. Males and females were analyzed separately, with n = 14 per genotype for males and n = 12–13 per genotype for females. (B) Latency to find a buried food item in Scn2a^E/+ mice compared with WT at 8 weeks of age. Latency to find a buried food item was not significantly different between Scn2a^E/+ and WT males (WT: 44.86 ± 11.58 s, Scn2a^E/+: 32.46 ± 9.95 s, P = 0.7471; Mann–Whitney test). Latency to find a buried food item was not significantly different between Scn2a^E/+ and WT females (WT: 89.00 ± 44.31 s, Scn2a^E/+: 161.7 ± 70.02, P = 0.2927; Mann–Whitney test). Symbols represent measured values from individual mice, horizontal lines represent mean and error bars represent SEM. Males and females were analyzed separately, with n = 13–14 per genotype for males and n = 13 per genotype for females.