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. 2022 Apr 11;31(17):2934–2950. doi: 10.1093/hmg/ddac085

Figure 5.

Figure 5

p.Asp943Gly variant of drsh-1 in C. elegans disrupts miRNA expression and causes heterochronicity. (A, C) Wild-type adult worm with vulva (dotted line) and gonad (arrowhead) highlighted. (B, D) Age-matched drsh-1(viz43) homozygous mutants that carry Individual 1’s variant (p.D943G in worm Drosha) show a heterochronic phenotype. At adulthood, drsh-1(viz43) mutants are reduced in size due to a failure to molt, yet they display the adult germline structure (dashed white line) and vulva (arrowhead) similar to wild type (C, D insets). Scale bars = 100 μm. (E) TaqMan assays measure the expression of miR-35 and let-7, known regulators of C. elegans development (19,107). Homozygous drsh-1(viz43) mutants as well as a null allele drsh-1(ok369) show reduced let-7 expression and no detectable miR-35 expression. Heterozygote data are presented as the drsh-1 allele over hT2G, a balancer chromosome.