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. 2022 Aug 18;13:953654. doi: 10.3389/fpls.2022.953654

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Copyright © 2022 Singh, Pillay, Naicker, Alexandre, Malatji, Mach, Steinkellner, Vorster, Chikwamba and Tsekoa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overlaid chromatogram of the HPLC-SEC analysis of the CAP256-VRC26 mAbs. HPLC-SEC chromatogram of the CAP256-VRC26.25 mAbs analyzed under non-reducing conditions. Chromatogram of CAP256-VRC26.25 mAbs produced in HEK293 (blue). CAP256-VRC26.25 coexpressed with (green) and without (red) CRISPR/Cas9 and sgRNA:NbCysP6 and sgRNA:NbVPE1a/b in N. benthamiana (ΔXTFT) and CAP256-VRC26.25 produced in HEK293 (blue).