Table 1.
HIV-1 neutralizing activity of the CAP256-VRC26.25 bNAbs produced in N. benthamiana (ΔXTFT) without/with Cas9/sgRNA coexpression.
| IC50 (μg/mL) | ||||
|---|---|---|---|---|
| CAP256-VRC26.25 | ||||
| Subtype | Envelope | HEK293-produced mAbs | N. benthamiana (ΔXTFT)-produced mAbs without Cas9/sgRNAs coexpression | N. benthamiana (ΔXTFT)-produced mAbs with Cas9/sgRNAs coexpression |
| C | Du422.01 | 0.11 | 0.10 | 0.20 |
| Du172.17 | >50 | >50 | >50 | |
| CAP210.E8 | 0.0012 | 0.0015 | 0.0014 | |
| CAP45.G3 | 0.0013 | 0.0014 | 0.0013 | |
| Du156.12 | 0.15 | 0.034 | 0.041 | |
| ZM197.7 | 0.11 | 0.056 | 0.098 | |
| ZM233.6 | 0.0044 | 0.0042 | 0.005 | |
| CAP214.15 | 3.12 | 4.31 | 2.77 | |
| B | QH0692.42 | >50 | >50 | >50 |
| TRO.11 | >50 | >50 | >50 | |
| 6535 | >50 | >50 | >50 | |
| A | Q461.e2 | 0.67 | 1.73 | 1.61 |
| Q168.a2 | 1.73 | 2.94 | 3.05 | |
| Q23.17 | 0.0014 | 0.0016 | 0.0016 | |
| Q842.d12 | >50 | >50 | >50 | |