TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| MC1061 | F−lac | 4 |
| BL21(DE3) | F−ompT (rB− mB−) prophage λ carrying T7 RNA polymerase gene | 39 |
| V. cholerae strains | ||
| TRH7000 | El Tor; Δ(ctxAB), wild type with respect to extracellular protein secretion | 11 |
| Mut8 | TRH7000 with Kmr insertion in epsL gene | 34 |
| A. hydrophila strains | ||
| Ah65 | Wild type | 4 |
| 89A | Kmr insertion in exeL gene | This study |
| Plasmids | ||
| pMMB207, pMMB208 | Broad-host-range cloning vectors; Cmr | 8, 22 |
| pMMB532 | epsM in pT7-5 | This study |
| pMS49 | epsL in pMMB208 | This study |
| pMS55 | exeL154epsL in pMMB208 | This study |
| pMS56 | epsL154exeL in pMMB208 | This study |
| pMS57 | exeL216epsL in pMMB208 | This study |
| pMS59 | exeL57epsL in pMMB208 | This study |
| pMS60 | epsL296exeL in pMMB208 | This study |
| pMS73 | epsL57exeL in pMMB208 | This study |
| pMS74 | exeL296epsL in pMMB208 | This study |
| pMS77 | epsE epsL in pMMB208 | This study |
| pMS79 | epsE exeL296epsL in pMMB208 | This study |
| pMS92 | epsL216exeL in pMMB208 | This study |
| pMS96 | exeE exeL154epsL in pMMB208 | This study |
| pMS97 | exeE exeL216epsL in pMMB208 | This study |
| pMS98 | epsE epsL154exeL in pMMB208 | This study |
| pMS99 | epsE epsL216exeL in pMMB208 | This study |
| pMS100 | epsE epsL296exeL in pMMB208 | This study |
| pMS101 | exeE exeL296epsL in pMMB208 | This study |
| pRJ84.1 | exeL in pMMB208 | This study |
| pT7-5 | T7 RNA polymerase promoter φ10, Apr | 40 |
| pWD600 | etxA etxB (E. coli heat-labile enterotoxin A and B subunit genes), Tcr | 5 |