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. 2022 Aug 18;13:963009. doi: 10.3389/fimmu.2022.963009

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Copyright © 2022 Ruan, Feng, Cheng, Wang, Zhang, Wu, Yang, Tian, Ou, Sun, Zhang, Mao, Zhu, Jia, Chen, Liu, Zhao, Huang, Gao, Yu, Zhang and Pan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pathogenicity of CHv-ΔgC/gE in ducklings. (A) Survival curves of ducklings after challenge with the indicated viral strains. (B) Daily body weight and temperature of all the ducklings after challenge with the indicated viral strains. (C) Postmortem examination of ducklings in each group at 5 dpi. (D) Histological analysis of heart, liver, spleen, duodenum, rectum and thymus from ducks injected with the indicated viral strains or MEM. Arrows indicate cellular infiltration or tissue disruption. (hematoxylin and eosin staining, 100× magnification). (E) Quantification of viral DNA loads in selected tissues with real-time PCR. Viral DNA copy numbers were determined with primers specific for UL30, as described above. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001; and ns, not significant.