Figure 4.

Recovery of deficient calcium signaling in astrocytes dampens neuronal hyperactivity

(A) Representative ΔF/F₀ calcium traces of neurons in App^NL control and App^NL-F mice expressing neuronal GCaMP6f (hSYN promoter) in addition to either control AAV (mCherry) or DREADDs in astrocytes (GFAP promoter). Scale bar, 20 μm.

(B) Heatmaps show neuronal calcium activity (ΔF/F₀ exceeding 2^∗SD baseline, y axis) over time (800 s, x axis) in App^NL control mice expressing mCherry (N = 5 mice, 463 neurons) or DREADDs (N = 4 mice, 640 neurons), and App^NL-F mice expressing mCherry (N = 5 mice, 652 neurons) or DREADDs (N = 5 mice, 466 neurons).

(C and D) Graphs show frequency (number of peaks per minute ± SEM) and signal amplitude (ΔF/F₀ ± SEM). ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, Kruskal-Wallis test with Dunn’s correction.