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. 2022 Aug 18;13:949670. doi: 10.3389/fimmu.2022.949670

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Copyright © 2022 Zhu, Kong, Li and Shao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of exosomes from Cynoglosussus semilaevis plasma. (A) Exosomes morphology viewed by transmission electron microscopy with both wide-field image (Top left) and close-up images (i-iii). i–iii denote the close-up images of exosomes with a diameter of ~100 nm at three different zones of the wild-field image. (C) Particle size distribution and concentration in the control (C6 and C96), early- (IN6 and IN16), and late-infection (IN48, IN72, IN96) groups analyzed by Nano-FCM. (B) Western blotting examination of exosome biomarkers (CD63, CD81, and HSP70) in control (C6 and C96) and infection (IN6, IN16, IN48, IN72, and IN96) groups.