Figure 5.

Host genetics and gut microbiota interact to modulate host body composition. (A) Association between body composition measures and α-diversity or first principal component (PC1) of β-diversity measured by mouse strains determined by using Spearman correlation. As indicated, red represents a positive correlation and blue represents a negative correlation. “***” = adj.p < 0.001, “**” = adj.p < 0.01, “*” = adj.p < 0.05, “.” = adj.p < 0.10, and “ns” = not significant. (B) Volcano plot of the differential genera abundance between C57BL/6J mice with high (above median) and low (below median) body fat percentage determined by ANCOM analysis. Structural zero represents the presence of the bacteria in one group and complete absence in another group. (C) Relative abundance of the Akkermansia in A/J mice with high and low body fat percentage. (D) Relative abundance of the Akkermansia in C57BL/6J mice with high and low body fat percentage. Mean abundance was compared using the Wilcoxon rank-sum test. For (C,D), “***” = P < 0.001 and “ns” = not significant. (E) Differential genera abundance between high (above median) and low (below median) body composition measures determined by ANCOM analysis. 20 most differentially abundant (based on cumulative ANCOM W-value) were selected for the graph. For easier presentation, ANCOM W values were converted to negative if CLR effect size is negative. Red indicates higher genera abundance in the above-median group and blue bacteria represents higher genera abundance in the below-median group. White represents a non-significant result obtained from ANCOM analysis. Models were adjusted for diet and sex as confounding factors. ANCOM models were FDR (BH-method) corrected for multiple comparisons and a significant sub-hypothesis test at a level of adj.P < 0.05 was counted toward W-value. The median was calculated for each mouse strain. For (E), “***” = ≥W_0.9, “**” = ≥W_0.8, “*” = ≥W_0.7, and “.” = ≥W_0.6.