Figure 7. Heterogeneity of Schwann cells and fate biasing in maturing peripheral nerves.

• A
  Dot plot of the top five differentially expressed genes from the milestones of the glial trajectory.
• B
  Left: selected cells (Foster et al, 2020) of the glial trajectory, middle: constructed tree overlayed on the UMAP, right: abstract dendrogram.
• C
  RNA velocity on tree‐derived clusters (milestones).
• D
  RNA velocity with CytoTRACE overlayed on the UMAP.
• E
  Dynamics of expression of known markers over the trajectory summarised over five bins per segment of the tree.
• F–H
  Heatmap of late markers of the three endpoints of differentiation from immature Schwann cells: endoneurial fibroblasts (F) and myelinating (G) or nonmyelinating Schwann cells (H).
• I
  Schematic representation of transitions from late “hub” cells biased to immature Schwann cells to all end fates along with mixed motor and sensory nerves.

Data information: enFibr, endoneurial fibroblasts; iSCs, immature Schwann cells; mSCs, myelinating Schwann cells; NMJs, neuromuscular junctions; nmSCs, non‐myelinating Schwann cells; SCPs, Schwann cell precursors; tSCs, terminal Schwann cells.