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. 2022 Aug 18;12:956940. doi: 10.3389/fonc.2022.956940

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Copyright © 2022 Iazzolino, Mendibil, Arnaiz, Ruiz-de-Angulo, Azkargorta, Uribe, Khatami, Elortza, Olalde, Gomez-Vallejo, Llop and Abarrategi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HC1806 cells show different cell behavior on HC1806 dECM compared to dECM from other tumor xenografts. (A) Detail of SEM imaging of samples showing surface structure (scale, 50 μm). (B-D) Images of cell-seeded samples. (B) Calcein-AM–positive cells are shown in green, while scaffold material is shown in grey (×20 (scale, 20 μm)). (C) Actin cytoskeleton immunostaining in orange; DAPI nuclear staining in blue (×10 (scale, 50 μm) and detailed images (scale, 20 μm)). (D) Ki67 immunostaining in green; DAPI nuclear staining in blue (×10 (scale, 20 μm) and detailed images (scale, 50 μm)). (E) Quantification of cell size (area) using actin cytoskeleton membrane distribution images. (F) Quantification of proliferative cells (Ki67-positive cells vs. total cells observed in each material). N = 3 in all cases and 2 areas measured per sample.