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Treatment type and concentrations used in each one of the decellularization protocols.
| Protocol | DNase (U/ml) | Freezing (cycles) | Trypsin (% w/v) | Triton X-100 (% w/v) | EDTA (% w/v) |
|---|---|---|---|---|---|
| a | – | 15 cycle | – | – | – |
| b | 50 U/ml | – | – | – | – |
| c | 50 U/ml | – | 0.25% | – | 0.25% |
| d | 50 U/ml | – | – | – | 2% |
| e | 50 U/ml | 15 cycle | – | – | 2% |
| f | 50 U/ml | – | – | 1% | – |
