Skip to main content
. 2022 Jul 11;12(9):jkac173. doi: 10.1093/g3journal/jkac173

Fig. 4.

Fig. 4.

Neither MEG-3/MEG-4 nor MEX-3 is required to promote condensation of PGL-1 or each other into RNP granules. a) RNAi of control lacZ or meg-3 meg-4 was done by feeding in fog-2 females with arrested oocytes. The MEG-3::mCherry strain was used as a positive control in each experiment to assess depletion of meg-3. The PGL-1::GFP, GFP::CGH-1, and GFP::MEX-3 reporters were used to assay condensation of the respective proteins. The intensity of fluorescence in granules is plotted on the Y-axes. b) RNAi of control lacZ or mex-3 was done by feeding in fog-2 females with arrested oocytes. The GFP::MEX-3 strain was a positive control in each experiment to assess depletion of mex-3. Reporter strains were used to assay condensation of PGL-1, MEG-3, and CGH-1. The intensity of fluorescence in granules, or the overall levels of fluorescence in oocytes (CTCF, see Methods), is plotted on the Y-axes. In all graphs, error bars are mean ± SEM. Statistical significance was determined using the Mann–Whitney test. **** indicates P < 0.0001, *** indicates P < 0.001, ** indicates P < 0.01, * indicates P < 0.05, and ns indicates not significant. n = 10–16.