Fig. 3.

Imaging-associated stress preferentially affects PGL-1 within large RNP granules of meiotically arrested oocytes. a) Micrographs of GFP-tagged RNA-binding proteins (MEX-3, CGH-1, PGL-1, MEG-3) in a fog-2 background. Top row: distribution of GFP in arrested oocytes early during imaging. Bottom row: distribution of GFP in arrested oocytes after extended imaging (late). Asterisk marks the most proximal oocyte in each germ line. Scale bar is 10 µm. b) Graphs showing either the number of GFP granules or the integrated density of GFP in granules in a single Z-slice of proximal oocytes (see Methods). Statistical significance was determined using the Mann–Whitney test. ** indicates P < 0.01 and ns indicates not significant. n = 18–33. Error bars indicate mean ± SEM.