Fig. 3.

Comparison of fertility between D. melanogastermh mutant females carrying mel-mh-Gfp vs. sim-mh-Gfp transgenes (single w mh⁶/w mh³¹; {mel-mh-Gfp, w⁺}attP40/+ vs . w mh⁶/w mh³¹; {sim-mh-Gfp, w⁺}attP40/+). Statistical significance between genotypes was tested by a 2 sample unpaired t-test: *** = 0.0001 < P ≤ 0.001; * = 0.01 < P ≤ 0.05; ns = P > 0.05 (not significant). a) Pilot experiment at room temperature (∼20–22°C). Females were collected as virgins and aged for either 3–4 days (left) or 9–10 days (right) prior to mating and progeny collection for 5 days. Progeny are reported as “per-day” to normalize with (b). b) Two large-scale experiments, performed at 25 and 27°C. Virgin females were aged for 3–5 days prior to mating. Crosses were flipped to fresh vials after 4 or 5 days, and again after an additional 4 or 5 days, for a total collection period of 12–13 days; the exact length of each flip was recorded and is accounted for in calculating the “progeny per day.” Note that vials were discarded if the parents died, which is why N goes down between flips (see Materials and Methods).