Table 2.

Testing mh transgenes for modulation of interspecific F1 hybrid viability

Set	Maternal genotype	# F1 hybrid daughters	# F1 hybrid sons	Ratio F1 daughters/sons	Relative ratio w/o and w transgene	P-Value
A	w	760	1106	0.69
	w; {mel-mh-Gfp}1029/+	766	1105	0.69	0.99	0.895
B	w	781	1144	0.68
	w; {mel-mh-Gfp}1048/+	830	1124	0.74	0.92	0.228
C	w	144	211	0.68
	w; {sim-mh-Gfp}1029/+	84	88	0.95	0.71	0.0722
D	w	248	529	0.47
	w; {sim-mh-Gfp}1048/+	461	693	0.67	0.70	0.000331

In the following descriptions, all flies are D. simulans unless noted otherwise. The designation {mh-Gfp, w⁺} refers to one of the 4 transgenic genotypes used in this table. The flies described in the “Maternal genotype” column were generated as follows: (1) w⁵⁰¹ virgin females were crossed to y w/Y; {mh-Gfp, w⁺} males. (2) w⁵⁰¹/y w; {mh-Gfp, w⁺}/+ virgin daughters were crossed to w⁵⁰¹/Y males. (3) For each set, y? w/w⁵⁰¹; {mh-Gfp, w⁺}/+ and y? w/w⁵⁰¹ virgin daughters were separately collected. The females from step (3) were then mated to D. melanogaster Canton-S males. The full genotypes of the transgenes are noted in the Materials and Methods. P-Values were calculated using a 2 × 2 Chi-squared test.