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. 2022 Jun 16;190(1):532–547. doi: 10.1093/plphys/kiac295

Figure 1.

Figure 1

Arabidopsis WRKY63 is a transcriptional activator and is involved in flowering regulation. A, The flowering phenotypes and rosette leaf numbers of WT and wrky63 mutants under LD and SD. **P < 0.001, *P < 0.01 (Student’s t test). Values are means ±sd. The experiments were performed with three biological replicates showing comparable results. B, Schematic structure of the effectors, reporter, and internal control used in the transient transcriptional activity assays. C, Transient transcriptional activity assays of WRKY63. GAL4BD-WRKY63 activated the expression of firefly LUCIFERASE (firefly LUC). Blank: no effector was added. GAL4BD was used as a negative control. GAL4BD fused with the activation domain VP16 (GAL4BD-VP16) was used as a positive control. Values are means ±sd. **P < 0.001 (Student’s t test). The experiments were performed with three biological replicates showing comparable results. RLU, relative luciferase activity.