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. 2022 Jun 16;190(1):532–547. doi: 10.1093/plphys/kiac295

Figure 3.

Figure 3

WRKY63 is induced by vernalization and is involved in vernalization-induced flowering. A, The flowering phenotypes and rosette leaf numbers of WT and wrky63 mutants under 0, 2, and 4 weeks of vernalization treatments in SD. At least 20 plants of each line were counted for the rosette leaf numbers at flowering. Values are means ±sd. **: P < 0.001 (Student’s t test). The experiments were repeated 3 times with comparable results. wV, weeks of vernalization. B, GFP fluorescence signal in WRKY63pro::WRKY63:GFP transgenic plants without vernalization (nV) or with 4 weeks of vernalization (+V). The experiments were performed with three biological replicates showing comparable results. Bar = 1 mm. C and D, Quantification of GFP fluorescent intensity (C) and the relative GFP fluorescent intensity (D) in WRKY63pro::WRKY63:GFP transgenic plants without vernalization (nV) or with 4 weeks of vernalization (+V). The X-axis represents the detected section of cotyledons, Y-axis fluorescence indicates the value of GFP intensity. Values of relative GFP intensity are normalized means ±sd. **P < 0.001. At least 10 leaves for each treatment were counted for the GFP intensity. E, Western blot of WRKY63pro::WRKY63:GFP in transgenic plants without vernalization (nV) or with 4 weeks of vernalization (+V). The western blots were performed with the indicated antibody, and the total protein stained with CBB was used as the loading control. F, The relative expression of WRKY63 in WT Arabidopsis without vernalization (nV) or with 4 weeks of vernalization (+V). **P < 0.001 (Student’s t test). Values are means ±sd. The experiments were performed with three biological replicates showing comparable results. CBB, coomassie brilliant blue.