Skip to main content
. 2022 Jun 11;190(1):516–531. doi: 10.1093/plphys/kiac278

Figure 3.

Figure 3

The effects of OsEPFL2 mutation on awn development. A, The gene framework of OsEPFL2 showing the coding region (rectangle), introns (horizontal lines), and CRISPR/Cas9 target sites (triangles). B, CRISPR/Cas9 editing of OsEPFL2 in Kasalath. The Kasalath image in Figure 3E is the same as that in Figure 1B. C, Amino acid alignment of Kasalath and OsEPFL2cas. D, Panicle comparison between kasalath, OsEPFL2cas1, and OsEPFL2cas2. Bar = 2 cm. E, Grains of Kasalath, OsEPFL2cas1, and OsEPFL2cas2. Bar = 0.5 cm. F and J, Grain width comparison between Kasalath, OsEPFL2cas1, and OsEPFL2cas2. Bar = 0.5 cm. G and K, Grain length comparison between Kasalath, OsEPFL2cas1, and OsEPFL2cas2. Bar = 0.5 cm. H, Awn length comparison between Kasalath, OsEPFL2cas1, and OsEPFL2cas2. I, The comparison of percentages of awned seeds between Kasalath, OsEPFL2cas1, and OsEPFL2cas2. L, Comparison of 1000-grain weight between Kasalath, OsEPFL2cas1, and OsEPFL2cas2. OsEPFL2cas1 and OsEPFL2cas2 are gene editing with CRISPR/Cas9 from Kasalath. OsEPFL2cas are short, or no awn. Values are mean ± sd, n = 10, different lowercase letters represent significant difference at 5% level according to least significant difference test.