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. 2022 Aug 18;16:917071. doi: 10.3389/fnins.2022.917071

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Copyright © 2022 Ghazale, Park, Vasan, Mester, Saleh, Trevisiol, Zinyk, Chinchalongporn, Liu, Fleming, Prokopchuk, Klenin, Kurrasch, Faiz, Stefanovic, McLaurin and Schuurmans.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ascl1^SA⁶ more efficiently represses astrocytic markers Sox9 and GFAP in transduced cortical cells compared to Ascl1. (A) Rosa-zsGreen cortex transduced with AAV5-GFAPlong-iCre, AAV5-GFAPlong-Ascl1-t2a-iCre, and AAV5-GFAPlong-Ascl1^SA⁶-t2a-iCre at 21 days post-transduction, showing zsGreen epifluorescence, Sox9 (red) and GFAP (white) expression in merged and separate channels. Blue is a DAPI counterstain. (B) Quantification of the percentage of zsGreen⁺ cells that co-express Sox9 or GFAP, and the percentage of zsGreen⁺Sox9⁺ cells that co-express GFAP. (C) Rosa-zsGreen cortex transduced with AAV5-GFAPshort-iCre, AAV5-GFAPshort-Ascl1-t2a-iCre, and AAV5-GFAPshort-Ascl1^SA⁶-t2a-iCre at 21 days post-transduction, showing zsGreen epifluorescence, Sox9 (red) and GFAP (white) expression in merged and separate channels. Blue is a DAPI counterstain. (D) Quantification of the percentage of zsGreen⁺ cells that co-express Sox9 or GFAP, and the percentage of zsGreen⁺Sox9⁺ cells that co-express GFAP. (E) Rosa-zsGreen cortex transduced with AAV8-GFAPshort-iCre, AAV8-GFAPshort-Ascl1-t2a-iCre, and AAV8-GFAPshort-Ascl1^SA⁶-t2a-iCre at 21 days post-transduction, showing zsGreen epifluorescence, Sox9 (red) and GFAP (white) expression in merged and separate channels. Blue is a DAPI counterstain. (F) Quantification of the percentage of zsGreen⁺ cells that co-express Sox9 or GFAP, and the percentage of zsGreen⁺Sox9⁺ cells that co-express GFAP. Scale bars = 100 μm. Significance was defined as p-values less than 0.05 and denoted as follows: ns, not significant, <0.05 *, <0.01 **, <0.001 ***.