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. 2022 Aug 18;12:939994. doi: 10.3389/fonc.2022.939994

Figure 4.

Figure 4

circ0038632 functions as a ceRNA by mediating DNMT3A in osteosarcoma. (A) Potential binding sites from 3′-UTR of DNMT3A with miR-186. (B) Relative luciferase activities of different co-transfected groups. (C) DNMT3A expression was tested after transfection by qRT-PCR. (D) The degree of circ0038632, DNMT3A, and miR-186 enrichment to Ago2 was examined by RIP assay. (E) The enrichment on Ago2 after transfection was tested. (F) DNMT3A mRNA expression was examined. (G) DNMT3A mRNA expression in osteosarcoma cells was tested. (H) DNMT3A mRNA expression in osteosarcoma tissues and its control were detected. (I) IHC staining of DNMT3A and Ki-67 were shown. **P < 0.01.