TABLE 1.
Purification of D. gigas SOD and catalase
| Enzyme | Purification step | Total protein (mg) | Total activity (U)a | Sp act (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| SOD | Crude extract | 105,600 | 3.60 × 105 | 3.4 | 100.0 | 0 |
| DE52-cellulose | 6,980 | 0.97 × 105 | 13.9 | 26.0 | 4 | |
| Hydroxylapatite | 2,000 | 0.40 × 105 | 20.0 | 11.0 | 41 | |
| DEAE Bio-Gel | 45 | 0.20 × 105 | 470.0 | 5.0 | 138 | |
| Superdex-75 | 3 | 0.60 × 104 | 1,900.0 | 1.6 | 559 | |
| Catalase | Crude extract | 35,862.0 | 1.9 × 106 | 52.6 | 100 | 0 |
| DE52-cellulose | 12,900.0 | 4.0 × 105 | 31.6 | 21 | 0.6 | |
| Q-Sepharose | 2,200.0 | 2.3 × 105 | 104.5 | 12 | 2 | |
| DEAE Bio-Gel | 328.0 | 1.2 × 105 | 384 | 6 | 7.3 | |
| Sephacryl S-300 | 36.8 | 1.0 × 105 | 2,728 | 5.3 | 52 | |
| Hydroxylapatite | 1.6 | 0.6 × 104 | 4,200 | 0.3 | 80 |
a
For SOD, 1 Unit of activity is defined as the amount of enzyme required to inhibit the rate of reduction of cytochrome c by 50% at 25°C. For catalase, 1 U of activity is defined as the amount of enzyme required for the decomposition of 1 μmol of H2O2 per min at 25°C.