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. 2022 Aug 18;13:985776. doi: 10.3389/fendo.2022.985776

Table 3.

Two-factor additive interaction between amino acids and Metformin.

OR (95% CI) P value
Additive interaction model of Glu and Metformin
Glu<98 and No-Metformin Reference
Glu<98 and Metformin 0.67 (0.38, 1.18) 0.159
Glu≥98 and No-Metformin 0.44 (0.27, 0.71) < 0.001
Glu≥98 and Metformin 0.61 (0.33, 1.13) 0.108
RERI 0.431 (0.077, 0.784)
APAB 0.262 (0.104, 0.420)
S 2.996 (0.693, 12.962)
Additive interaction model of Gly and Metformin
Gly<196 and No-Metformin Reference
Gly<196 and Metformin 0.77 (0.47, 1.27) 0.299
Gly≥196 and No-Metformin 0.46 (0.29, 0.75) 0.001
Gly≥196and Metformin 0.57 (0.34, 0.98) 0.036
RERI 0.463 (0.102, 0.824)
APAB 0.275 (0.119, 0.432)
S 3.101 (0.695, 13.824)
Additive interaction model of Leu and Metformin
Leu<128 and No-Metformin Reference
Leu <128 and Metformin 0.85 (0.54, 1.34) 0.485
Leu≥128 and No-Metformin 0.48 (0.29, 0.8) 0.004
Leu≥128 and Metformin 0.71 (0.44, 1.15) 0.153
RERI 0.376 (0.0753, 0.676)
APAB 0.263 (0.102, 0.424)
S 8.168 (0.005, 12833.36)
Additive interaction model of His and Metformin
His<51 and No-Metformin Reference
His <51 and Metformin 0.88 (0.56, 1.37) 0.559
His≥51 and No-Metformin 0.46 (0.29, 0.75) 0.001
His≥51 and Metformin 0.73 (0.45, 1.17) 0.181
RERI 0.458 (0.097, 0.818)
APAB 0.273 (0.118, 0.429)
S 3.100 (0.707, 13.580)
Additive interaction model of Phe and Metformin
Phe<47 and No-Metformin Reference
Phe <47 and Metformin 0.83 (0.54, 1.3) 0.418
Phe≥47 and No-Metformin 0.24 (0.14, 0.42) < 0.001
Phe≥47and Metformin 0.72 (0.46, 1.15) 0.166
RERI 0.427 (0.090, 0.764)
APAB 0.275 (0.117, 0.432)
S 4.377 (0.294, 65.066)
Additive interaction model of Tyr and Metformin
Tyr<47 and No-Metformin Reference
Tyr <47 and Metformin 0.81 (0.53, 1.26) 0.351
Tyr≥47 and No-Metformin 0.41 (0.24, 0.68) < 0.001
Tyr≥47 and Metformin 0.71 (0.45, 1.12) 0.138
RERI 0.384 (0.067, 0.701)
APAB 0.256 (0.094, 0.418)
S 4.328 (0.213, 87.960)

Glu Glutamate, Gly Glycine, Leu Leucine, His Histidine, Phe Phenylalanine, Tyr tyrosine, OR odds ratio, CI confidence interval, RERI risk due to interaction, AP attributable proportion due to interaction, S synergy index.

Multivariable Model was adjusted for age, gender, body mass index, systolic blood pressure, diastolic blood pressure, triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, glycosylated hemoglobin, uric acid, serum creatinine.

Significant elative excess risk due to two of interaction (RERI) > 0, attributable proportion due to interaction (AP) > 0 or synergy index (S) > 1 indicates a significant additive interact.