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. 2022 Sep 1;22:424. doi: 10.1186/s12870-022-03802-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Functional verification of PmWRKY31-overexpressing transgenic tobacco. a Phenotypic changes in tobacco under drought stress. b Phenotypes of WT tobacco and the transgenic W and W3 lines at 60 d of normal culture. c Phylogenetic tree analysis of WRKY genes in different species. The blue triangle represents PmWRKY31. d Changes in the contents of MDA and Pro under drought stress. 0 d, 2 d, and 4 d represent the drought stress treatment time. WT, W, and W3 represent wild-type tobacco and the W and W3 lines of transgenic tobacco, respectively. Different lowercase letters indicate significant differences at P < 0.05