Fig. 5.

Validation of Map4k4 as a target gene of miR-547-3p. A Prediction of binding sites between miR-547-3p and Map4k4. B Luciferase reporter gene assay was performed to validate the interaction between miR-547-3p and Map4k4. The data are expressed as mean ± SD (N = 3) and analyzed for statistical significance using one-way ANOVA followed by LSD post hoc analysis for multiple comparisons (^**P < 0.01 versus miRNA-NC + Map4k4-WT group). C, D Western blot and qRT-PCR analysis show the effects of miR-547-3p mimics or inhibitor on the expression of Map4k4 in the DRG cells. The data are expressed as mean ± SD (N = 3) and analyzed for statistical significance using one-way ANOVA followed by LSD post hoc analysis for multiple comparisons (^**P < 0.01 versus control group). DRG dorsal root ganglion, LSD least significant difference, NC negative control, WT wild-type, MUT mutant, qRT-PCR real-time quantitative reverse transcription PCR, SD standard deviation