Figure 5.
MiRNA-31-5p inhibits SP-induced autophagy in PAECs through targeting NAT8L. (A) The putative binding site of miRNA-31-5p in 3′-UTR of NAT8L. (B) Relative luciferase activity in PAECs co-transfected with mutant/wild-type plasmids containing the putative binding site of miRNA-31-5p in NAT8L and miRNA-31-5p mimic. (C and D) Effect of miRNA-31-5p mimic and inhibitor on the protein expression of NAT8L in control PAECs. (E) Effect of miRNA-31-5p mimic and inhibitor on the protein expression of NAT8L in control PAECs treated with SP (10 µM). (F) Protein expression of NAT8L in PAECs transfected with pcDNA3.1-GAS5 and the combination of pcDNA3.1-GAS5 and siRNA-GAS5, as assessed by Western blotting. *P<0.05. There were at least three replicates in each group available for analysis. SP, spermidine; PAECs, pulmonary artery endothelial cells.