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. 2022 Aug 2;26(4):297. doi: 10.3892/mmr.2022.12813

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — GAS5/miRNA-31-5p/NAT8L axis is associated with SP-induced autophagy in PAECs in vivo. (A) Representative hematoxylin and eosin staining images of proximal pulmonary vascular tissues. (Scale bar, 20 µm; magnification, ×40). (B) Expression of GAS5 in PAECs of CTEPH rats treated with SP and the combination of SP and siRNA-GAS5. (C) Expression of miRNA-31-5p in PAECs of rats with CTEPH treated with SP and the combination of SP and siRNA-GAS5. (D) Protein expression of NAT8L in PAECs of rats with CTEPH treated with SP and the combination of SP and siRNA-GAS5. (E) The percentage of GFP-LC3 positive PAECs as assessed by GFP-LC3 adenovirus assays. (Scale bar, 50 µm; magnification, ×20). *P<0.05. There were at least three replicates in each group available for analysis. GAS5, growth arrest-specific transcript 5; SP, spermidine; PAECs, pulmonary artery endothelial cells; siRNA, small interfering RNA; CTEPH, chronic thromboembolic pulmonary hypertension.