Figure 6. TCM persist longer in the circulation, are skin tropic and replenish other memory T cell subsets.
(A-G) Circulating memory T cell precursor populations were analyzed by mass cytometry for the expression of cutaneous lymphocyte antigen (A), chemokine receptors (B), HLA-DR (C), BCL-2 (D), exhaustion markers PD-1 and Tim-3 (E,F) and Ki-67 (G). The mean and SEM of results from six different blood donors are shown. (H,I) TCM persist longer in the circulation. T cells from the blood and spleen of injected mice three weeks after infusion were analyzed by flow cytometry. The mean and SEM of six experiments using six different blood donors are shown. (I) TCM replenished other T cell subsets. The phenotype of T cells in blood three weeks after T cell infusion is shown. The mean and SEM of experiments using three different blood donors are shown. Significance was determined by a one-way ANOVA and Tukey’s post-hoc test for multiple comparisons.