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. 2000 Feb;182(3):833–836. doi: 10.1128/jb.182.3.833-836.2000

FIG. 2.

FIG. 2

Decay of P. mirabilis FlhD and FlhC proteins after translation arrest. (A) Immunoblot of extracts from cells harvested at peak differentiation (3.5 h on seeding plates). Cells were immediately resuspended in LB to an A600 of 1.0, and translation was blocked by addition of 200 μg of spectinomycin per ml. Samples were taken and transferred immediately (time zero) and after 15 and 30 min of incubation at 37°C into 10% (final concentration) trichloroacetic acid and cen- trifuged, and the pellets were washed with 80% acetone and resuspended in loading buffer. (B) Decay of P. mirabilis FlhD and FlhC proteins in the early and postdifferentiation periods (2 h 45 min and 4 h 15 min, respectively). Mean values were derived from three experiments carried out as described for panel A, by scanning of immunoblot signal strengths (Kodak Digital Science, NIH Image 1).

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