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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Methods Mol Biol. 2022;2482:169–179. doi: 10.1007/978-1-0716-2249-0_11

Figure 1: Transient transfection and luciferase reporter assays using primary mouse hepatocytes.

Figure 1:

A) Ectopic expression of EGFP in primary hepatocytes. B) Results of luciferase reporter assay in primary mouse hepatocytes by transient transfection. Hepatocytes were transfected with pGL4-Basic control plasmid or Bmal1-Luc reporter, with or without ectopic expression of the transcriptional repressor, Rev-erbα. Luminescence units were normalized to LacZ and labeled as RLU. Data represent the mean ± SEM. *** indicates p < 0.001 by Student’s t-test.