Fig. 1.

Glioblastoma cell atlas before, during, and after treatments. (A) Workflow for new and recurrent glioblastoma processing and analysis. (B) First presentation, postoperative, and at recurrence brain imaging, treatment timeline, and progression-free survival for patients with recurrent glioblastoma. Asterisks mark the original tumor site. Circles mark the recurrent tumor site. Patients either recurred during or shortly after adjuvant treatment (less than 12 months from surgery) or later (more than 18 months from surgery). (C) Copy-number anomalies for all patients separate glioblastoma cancer cells and non-cancer cells. Cancer cells contain characteristic anomalies in chromosome 7 and/or 10 in all patients. Non-cancer cells do not contain chromosome-wide anomalies. (D) tSNE visualization of all cancer cells from glioblastoma samples. Cells are colored according to the sample. Abbreviations: cNMF, clustered non-negative matrix factorization; post-op, postoperative; pre-op, preoperative; TME, tumor microenvironment; tSNE, t-distributed stochastic neighbor embedding.