Fig. 4.
Single-cell analysis of the adult human subventricular zone. (A) Schematic showing the workflow and analysis of the SVZ cells. (B) Magnetic resonance imaging of each SVZ of the patient demonstrating the regions of signal abnormality and the distant site (arrowheads) where the sample was derived. (C) tSNE of SVZ cells from all patients colored by cell type. (D) Heatmap of gene expression by cell type. (E) tSNE with cells colored according to the expression of selected progenitor genes. (F) Matrix of similarity between NSC signatures and the fetal signature.3 (G) CNA heatmap of SVZ cells. (H) tSNE and box plot of SVZ cells from all patients with cells harboring chromosome 7 amplification and chromosome 10 deletions identified. tSNE: CNA-harboring cells are red. Boxplot shows the percentage of CNA-harboring cells per sample, stratified by sample type. Only samples from patients with IDHwt glioblastoma contain CNA-harboring cells. P-value: *<.05, one-sample Student’s t test. (I) tSNE of SVZ with cells labeled according to patient. (J) Matrix of similarity between NSC signatures and cancer meta-programs. (K) Matrix of similarity between cancer meta-programs and TME/SVZ neural cell type signatures. (L) Matrix of similarity between cancer meta-programs and fetal signatures in (F). Abbreviations: APC, astrocytic progenitor cell; Astro, astrocytic; CAN, copy number alteration; EN, excitatory neuron; ENP, excitatory neuronal progenitor; GPC, glial progenitor cell; IN, interneuron; IPC, inhibitory neuronal progenitor cell; Mes, mesenchymal; Neuro, neuronal; NSC, neural stem cell; Oligo, oligodendrocytic; OPC, oligodendrocyte progenitor cell; RG, radial glia; SVZ, subventricular zone; TME, tumor microenvironment; tRG, truncated radial glia; tSNE, t-distributed stochastic neighbor embedding.