Table 1.

Patient with Glioblastoma and Sample Information

Sample	Age	Sex	Location	IDH Mutational Status	MGMT Promoter Methylation Status	Time to Recurrence (Months)	Site of Recurrence	No. of Cells	Median Genes per Cell	Median UMI per Cell	%TME
+BT333	68	M	Right temporal, corpus callosum	WT	Unmethylated			614	2561	6800	NA
+BT338	38	M	Right frontal, temporal	WT	Methylated			1404	4227	14 060	NA
+BT346	58	M	Right frontal, insular	WT	Methylated			1902	3472	11 332	NA
+BT363	58	M	Left frontal	WT	Unmethylated			9784	2088	5001	11.9
+BT364	64	M	Left parietal, occipital	WT	Methylated			6676	2852	8729	23.2
+BT368	77	F	Left temporal	WT	Methylated			2478	2688	7558.5	9.8
BT373	62	M	Right temporal, insular	WT	Unmethylated			6590	1181	2486.5	20.9
BT377	51	M	Right temporal, insular	WT	Methylated			3877	2173	6896	77
BT380	68	M	Right frontal, insular	WT	Unmethylated			4228	1977	7210	73.0
+BT389	60	M	Left temporal, insular	WT	Methylated			4166	1673	4801	75.0
+BT390	82	M	Right temporal, occipital	WT	Methylated			3440	1722	4469.5	68.0
+BT397	60	M	Left frontal	WT	Unmethylated			5764	1889	5988.5	90.2
+BT400	63	F	Left temporal	WT	Unmethylated			4969	1434	2772	9.4
+BT402	66	F	Right frontal, parietal	WT	Methylated			4856	1743	5017	78.1
+BT407	54	M	Right frontal, temporal	WT	Unmethylated			3602	1838	6242.5	97.0
+BT409	68	M	Right temporal, parietal, occipital	WT	Methylated			2267	1761	4732	57.9
BT420	57	F	Right temporal, insular	WT	Unmethylated			2283	2719	7794	13.5
BT356	75	F	Right Frontal	WT	Methylated	19	Distant	7577	2197	6385	4.8
BT369	74	F	Left Temporal	WT	Unmethylated	18	Local	3586	836	1456.5	46.0
BT374	57	F	Right temporal	WT	Unmethylated	20	Local	2374	3328	10 522	23.7
BT389	58	M	Right parietal, temporal, occipital	WT	Unmethylated	7	Local	4672	2388	6715	89.0
BT396	54	M	Left parietal	WT	Not determined	86	Distant	3680	1633	3765	83.3
BT408	54	F	Right temporal	WT	Unmethylated	7	Local	3045	1569	4138	96.0
BT419	74	M	Left temporal	WT	Unmethylated	11	Local	4148	1260	2793	96.2
BT426	55	M	Left temporal	WT	Unmethylated	7	Local	4123	833	1854	86.7

Abbreviations: IDH, isocitrate dehydrogenase; MGMT, O(6)-methylguanine-DNA methyltransferase; NA, not applicable; TME, tumor microenvironment; UMI, unique molecular identifiers; WT, wild type.

Samples marked with + were taken from Couturier et al³; samples with NA under %TME were depleted for TME before scRNA-seq.