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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Cell Stem Cell. 2020 May 7;26(5):755–765.e7. doi: 10.1016/j.stem.2019.12.006

Figure 1. LSK and MP cells exhibit unique translational programs.

Figure 1.

(A) Schematic of cell populations evaluated (top). Representative polysome profiles from LSK (Lin−Sca-1+c-Kit+) and MP (Lin−Sca-1−c-Kit+) cells. 28S and 18S ribosomal RNA bands from total RNA prepared from each fraction are shown. Polysome/subpolysome RNA ratios (poly/subpoly) were calculated by dividing total RNA from polysomes (fractions 5–10) by total RNA in subpolysomes (fractions 1–4) (n=3, p < 0.05). (B) Comparison of differentially expressed genes based on total RNA or TE. More differentially expressed genes are highly expressed in LSK compared to MP cells based on total RNA (top). Breakdown of differential gene expression based on TE (bottom): high TE in LSK (green), high TE in MP (blue), no difference in TE or protein expression between LSK and MP cells (gray). (C) Integration of RNA-seq and whole proteome data. Differentially expressed genes based on total RNA-seq and protein expression in LSK versus MP cells (top panel). Number of genes in each quadrant is indicated and also expressed as a percentage of all differentially expressed genes identified. Comparison of TE to protein expression in LSK and MP cells for group II and IV genes in the top panel (bottom panel). (D) Heatmap showing pathways significantly enriched in LSK or MP cells based on TE. (E) Enrichment plots for pathways enriched in LSK cells based on TE (left) or total RNA expression (right).