Table 1. Kinetic constants determined by biolayer interferometry.
The association and dissociation constants, k on and k off, respectively, are shown as well as the overall K d (k off/k on) value. The goodness of fit of the binding data to a 1:1 Langmuir model is represented by the R 2 or coefficient of determination. Each experiment was run in triplicate. N.D., not determined due to negligible dissociation.
| RBD | k on × 105 (M −1 s−1) | k off × 10−4 (s−1) | K d (nM) | R 2 |
|---|---|---|---|---|
| wt | 1.3 ± 0.3 | 11.9 ± 5.2 | 8.9 ± 2.1 | 0.9980 |
| Q498Y | 1.6 ± 0.3 | 4.5 ± 2.5 | 3.0 ± 2.2 | 0.9975 |
| β | 1.2 ± 0.1 | 2.9 ± 0.4 | 2.4 ± 0.5 | 0.9976 |
| K417N | 1.0 ± 0.2 | 13.7 ± 1.7 | 14.6 ± 3.8 | 0.9968 |
| E484K | 1.3 ± 0.4 | 9.3 ± 5.7 | 6.8 ± 2.4 | 0.9967 |
| N501Y | 1.2 ± 0.1 | N.D. | N.D. | 0.9973 |
| Q493K+Q498Y+P499T | 1.3 ± 0.1 | 3.4 ± 0.7 | 2.7 ± 0.8 | 0.9972 |