Table 6. Effect of temperature on the binding kinetics of RBD Q498Y to human ACE2.

The affinity K _d values for the binding of ACE2 to plate-immobilized RBD Q498Y are shown. For purposes of comparison, the binding was also tested with RBD wt and RBD β.

K d (nM)	4°C	RT	37°C
WT	17	29	58
Q498Y	9	12	16
β	14	25	73